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Formate Colorimetric Assay Kit

Catalog #: K653

In stock

$455.00

Product Details

Cat # +Size K653-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications The assay can detect formate in the range from 0.5 to 10 nmols/well.
Features & Benefits • Simple procedure; takes ~60 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of formate levels in a number of sample types
Kit Components • Formate Assay Buffer
• Formate Enzyme Mix
• Formate Substrate Mix
• Formate Standard (100 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Formic acid (HCOOH) is the simplest carboxylic acid and occurs naturally, notably in ant and bee venom. It is normally present at low levels in blood and urine (up to ~ 0.5 mM) but can be present in concentrations as high as 5 mM in acute methanol poisoning. Environmental exposure to formaldehyde can also elevate the blood and urine levels of formate. In the Formate Assay Kit, formate is oxidized to generate a product resulting in color formation (λ = 450 nm) proportional to formate concentration. The kit provides a convenient means for detecting formate in biological samples such as in serum or plasma, cells, culture and fermentation media. There is no need for pretreatment or purification of samples.


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Can this kit be used with samples like bacteria, plants, drosophila, yeast etc?
We have optimized the kit with mammalian samples. However, theoretically this kit should work with samples from multiple species/sources. Formate is not species specific.
Can samples with reducing agents like DTT be used with this kit?
In the kit, formate is oxidized to generate a colored product. So DTT or any reducing agent is not advisable.
What compounds in the cell lysate can interfere in the assay?
NAD(P)H in samples may generate background readings. If significant amount of NAD(P)H is present in your sample, a sample background control can be performed by omitting Enzyme Mix in the Reaction Mix, then the sample background reading can be subtracted from the sample reading.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
Can this assay be normalized with respect to protein concentration?
Yes, a detergent-compatible BCA assay can be used for protein quantitation to normalize sample amount.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.