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Folic Acid ELISA Kit

A competitve ELISA kit for the quantitative measurement of serum, urine and tissues (e.g. pork, liver, chicken, fish and shrimp)

WARNING: This product can expose you to chemicals including TMB, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: E4523
$670.00

Product Details

Cat # +Size E4523-100
Size 100 assays
Kit Summary • Detection method- Absorbance (450 nm)
• Species reactivity- Universal
• Application- quantitative measurement of folic acid in serum, urine and tissues (e.g. pork, liver, chicken, fish and shrimp).
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications This ELISA kit is used for quantitative measurement of folic acid in serum, urine and tissues (e.g. pork, liver, chicken, fish and shrimp)
Features & Benefits • This ELISA kit is used for in vitro quantitative determination of folic acid.
• Detection Range: 2 - 250 ng/ml
• Detection limit: 50 pg/well (1 ng/ml)
• Cross Reactivity: Pefloxacin – 0%, Kanamycin – 0%
Kit Components • ELISA Microplate
• Folic Acid Standard
• HRP-conjugate
• Antibody
• TMB substrate
• Stop Solution
• Sample Diluent
• Wash Buffer (10X)
• Serum Solution
• Standard Buffer
• Plate Sealers
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Folic acid (also called folate or vitamin B9) belongs to one of the antioxidative water-soluble B vitamins. Folic acid is an essential vitamin and human need to get it from the diet. It is naturally abundant in many foods but it is particularly enriched in dark green vegetables and liver. The main functions of folic acid are to synthesize nucleic acids and metabolize amino acids for cell division. Folic acid is also suggested to be critical for promoting fertility and preventing heart diseases. Folate deficiency may cause diarrhea, depression, confusion, anemia, and fetal neural tube defects. The traditional techniques/instruments (HPLC or GC-MS) for detecting folic acid are complex, expensive, laborious, and time-consuming. Immunoassay techniques, such as ELISA, are commonly preferred as a simple, reliable and rapid methods. BioVision’s Folic Acid ELISA Kit is a competitive-based ELISA that detects folic acid in tissues, urine and serum. This detection kit offers ready-to-use reagents, and can quantify a broad range of folic acid (2 – 250 ng/ml) within 90 minutes.


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My sample is human RBC. Is it compatible with this assay? Can I use other cells with this assay?
The kit is not compatible with RBCs. We have not tested other cells, but theoretically it should be possible to use cells for this assay. Please prepare the cell lysate just as described in the data sheet for tissue samples. Homogenize 1 x 10^6 cells in 200-500 µl of sample diluent. Vortex for 5 min. Centrifuge the samples at 10,000 g at 4°C for 15 min and take the supernatant. Use the supernatant for the assay. 1 x 10^6 million cells/sample is recommended starting point. The user will have to optimize the cell number based on the cell type.
Is the kit specific for unmetabolized folic acid or does it also recognize metabolic derivatives such as dihydrofolates, 5-methyltetrahydrofolate (5-MTHF) and tetrahdrofolic acid ?
Our Folic Acid Elisa Kit quantifies the unmetabolized Folic Acid. We have not tested the cross reactivity with other metabolic derivatives of Folic Acid such as DHF or 5MTHF etc. Since we have not tested, we do not know whether it detects these derivatives.