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FNDC5 (Human) ELISA Kit

A Sandwich ELISA kit for the quantitative measurement of human FNDC5 in serum, plasma and other biological fluids.

WARNING: This product can expose you to chemicals including Dimethylformamide (DMF), which is known to the State of California to cause cancer. For more information go to
Catalog #: E4860

Product Details

Cat # +Size E4860-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications • in-vitro quantitative determination of Human FNDC5 concentrations in serum, plasma and other biological fluids.
Features & Benefits • Precision: Coefficient of variation is < 10%
• Sensitivity: 0.10 ng/mL
• Detection Range: 0.16-10 ng/mL
• Specificity: This kit recognizes Human FNDC5 in samples. No Significant cross-reactivity or interference between Human FNDC5 and analogues was observed.
Kit Components • Micro ELISA Plate
• Reference Standard
• Biotinylated Detection Ab (100x)
• HRP Conjugate (100x)
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer (25X)
• Substrate Reagent
• Stop Solution
• Plate Sealer
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Fibronectin Type III Domain Containing 5 (Irisin) is a newly discovered hormone, secreted into circulation by muscle, which acts on white adipocytes and promotes WAT “browning”. Irisin can reduce body weight and cause induction of brown adipocyte in vivo, including a capacity for thermogenic energy expenditure mediated by uncoupling protein 1 (UCP-1). Irisin mature protein is comprised of 110 amino acid residues and two potential glycosylation sites. BioVision’s FNDC5 (Human) ELISA Kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FNDC5. Standards or samples are added to the micro ELISA plate wells that bind to the specific antibody. Then a biotinylated detection antibody specific for Human FNDC5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The wells are washed, a TMB substrate solution is added to the wells and blue color develops in proportion to the amount of FNDC5 bound. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human FNDC5. The concentration of Human FNDC5 in the samples can be calculated by comparing the OD of the samples to the standard curve.

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