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Fluo-4 AM

A membrane permeable fluorescent calcium indicator
Catalog #: B3146
SKU-Size Size Price Qty
B3146-5PK 5 x 50 μg
B3146-500 500 μg
More Sizes Get Quote

Product Details

Alternate Name Calcium (Ca2⁺) indicator; Fluo-4 membrane permeable; Fluo-4 acetoxymethyl ester; Fluorescent Calcium indicator
Appearance Red orange powder
CAS # 273221-67-3
Molecular Formula C₅₁H₅₀F₂N₂O₂₃
Molecular Weight 1097
Purity ≥ 95%
Solubility ~1 mg/ml in DMSO
InChi InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-
PubChem CID 4060965
Handling Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Fluo-4 is a widely used green fluorescent, intracellular calcium (Ca²⁺) indicator. Fluo-4 AM is the membrane permeable form of the calcium indicator. Masking the negative charge using non-polar, ester-linked moieties (AM ester) allows the molecule to enter cells through passive diffusion. Once inside the cell, ubiquitous intracellular esterase enzymes promote rapid hydrolysis of the AM ester leaving the active, highly polar form of the indicator trapped inside the cell. It binds to calcium with a Kd value of 355 nM and displays excitation/emission maxima of 490/515 nm, respectively. It is used to measure intracellular Ca²⁺ flux in high throughput screening and fluorescence microscopy applications.

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