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FLT3 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of FLT3 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5006
$750.00

Product Details

Cat # +Size E5006-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of FLT3 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Detection range: 0.313 – 20 ng/ml
● Sensitivity: 0.188 ng/ml
● Recovery range: 85 - 105% for normal human serum and plasma samples
● This Sandwich ELISA is highly sensitive and highly specific for the detection of FLT3 in human samples. There is no significant cross-reactivity or interference between FLT3 and analogues
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (20 ng)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

FMS-like tyrosine kinase 3 (FLT3, CD135) is a transmembrane ligand-activated receptor protein that belongs to the receptor tyrosine kinase class III. It is expressed on the surface of multiple hematopoietic stem and progenitor cells. FLT3 serves as an efficient cell surface marker to predict the type of cell, differentiation stage, and cell activity. FLT3 also regulates cell survival, proliferation, and differentiation via various signaling pathways such as PI3K, RAS, and STAT. Mutations of FLT3 are associated with acute myeloid leukemia (AML) and, hence FLT3 could be an efficient prognostic marker to predict the adverse outcome of the disease. FLT3 inhibitors such as Gilteritinib, Midostaurin, Sorafenib have demonstrated considerable success in the treatment of AML patients positive for FLT3. BioVision’s FLT3 (Human) ELISA kit quantitatively measures FLT3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FLT3 in the sample or standard.


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