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Product Details
| Cat # +Size | E4970-100 |
|---|---|
| Size | 96 assays |
| Detection Method | Absorbance (450 nm) |
| Species Reactivity | Human |
| Applications | Sandwich ELISA kit to quantitatively measure FLG in human serum, plasma, tissue lysate, and other biological fluids |
| Features & Benefits | ● This Sandwich ELISA is highly sensitive and highly specific for the detection of FLG in human samples. There is no significant cross-reactivity or interference between FLG and analogues ● Recovery range: 85 - 105% for normal human serum and plasma samples ● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10% ● Detection range: 0.313 – 20 ng/ml ● Sensitivity: 0.188 ng/ml |
| Kit Components | ● Micro ELISA plate ● Wash Buffer (25X) ● Plate Sealers ● Standard (Lyophilized) (20 ng) ● Sample/Standard Dilution Buffer ● Biotin-labeled Antibody ● Antibody Dilution Buffer ● HRP-Streptavidin Conjugate (SABC) ● SABC Dilution Buffer ● TMB Substrate Solution ● Stop Solution |
| Storage Conditions | 4ºC |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Filaggrin (FLG) is a critical structural protein in the stratum corneum of the skin epidermis. The protein is synthesized as a precursor (Profilaggrin) and is localized in keratohyalin granules in the stratum granulosum. During terminal differentiation of epidermal cells, Profilaggrin undergoes proteolysis to yield monomeric filaggrin units. Filaggrin regulates epidermal homeostasis. It also maintains skin barrier function. Additionally, it promotes the aggregation of keratin intermediate filaments to form a keratin network which contributes to the physical strength of the skin. Various proteases hydrolyze Filaggrin to yield amino acids and amino acid derivatives that function as natural moisturizing factors in the stratum corneum. Mutations in Filaggrin result in skin disorders such as atopic dermatitis and eczema. Autoantibodies in rheumatoid arthritis (RA) recognize citrullinated residues in Filaggrin. Hence antibodies to Filaggrin are used as markers to detect RA. BioVision’s Filaggrin (Human) ELISA kit is used to detect Filaggrin in human serum, plasma, and other biological fluids. The kit is based on the Sandwich principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of Filaggrin in the sample or standard.
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