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FGF9 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of FGF9

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5134
$750.00

Product Details

Cat # +Size E5134-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of FGF9 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Highly sensitive and specific
● Detection range: 62.5-4000 pg/ml
● Recovery Rate: 87-103% for serum, 85-104% for EDTA plasma and 87-100% for heparin plasma
● Sensitivity: 37.5 pg/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (4000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

FGF9 also known as Fibroblast growth factor 9 belongs to the fibroblast growth factor (FGF) family, which plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF family members possess broad mitogenic and cell survival activities. FGF has an essential role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors. FGF9 has also been shown to play a vital role in male sex development. FGF9’s role in sex determination begins with its expression in the bi-potent gonads for both females and males. The absence of Fgf9 causes an individual, even an individual with X and Y chromosomes, to develop into a female, as it is needed to carry out important masculinizing developmental functions such as the multiplication of Sertoli cells and creation of the testis cords. Overexpression of FGF9 in prostate epithelial cells can lead to high grade prostate intraepithelial neoplasia, which is a precursor for prostate cancer. BioVision’s FGF9 (Human) ELISA Kit is used for the quantitative detection of FGF9 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FGF9 in the sample.


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