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FGF7 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of FGF7

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5133
$750.00

Product Details

Cat # +Size E5133-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of FGF7 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Detection range: 31.25-2000 pg/ml
● Recovery Rate: 85-105% for serum, 86-103% for EDTA plasma and 90-102% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
● Sensitivity: 18.75 pg/ml
● Highly sensitive and specific
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (2000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

FGF7 also known as Fibroblast growth factor 7 belongs to the fibroblast growth factor (FGF) family, which plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF family members possess broad mitogenic and cell survival activities. FGF is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and normal branching morphogenesis. Transgenic expression of FGF7 in vivo led to the induction of cells with characteristics of liver progenitor cells and ameliorated hepatic dysfunction. FGF7 as a potential therapeutic target for liver diseases. FGF7 also plays an important role in the stroma-epithelium interactions that take place in several tissues, including the prostate and mammary glands. BioVision’s FGF7 (Human) ELISA Kit is used for the quantitative detection of FGF7 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FGF7 in the sample.


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