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FGF6 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of FGF6

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to
www.P65Warnings.ca.gov.
Catalog #: E5143
$750.00

Product Details

Cat # +Size E5143-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of FGF6 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Recovery Rate: 87-104% for serum, 87-105% for EDTA plasma and 85-104% for heparin plasma
● Detection range: 93.75-6000 pg/ml
● Highly sensitive and specific
● Sensitivity: 56.25 pg/ml
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (6000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

FGF6 also known as Fibroblast growth factor 6 belongs to the fibroblast growth factor (FGF) family, which are glycosaminoglycan binding proteins which possess broad mitogenic and cell survival activities. These are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. It plays an important role in the regulation of cell proliferation, cell differentiation, angiogenesis and myogenesis. Fgf6 is the only known member of the FGF family whose expression is restricted to the muscle cell lineage during development, suggesting it may have a role in myogenesis. Intramuscular FGF6 concentrations could influence the proliferation and differentiation processes taking place during development. FGF19 is required for normal muscle regeneration. FGF6 is increased in high-grade prostatic intraepithelial neoplasia and prostate cancer and can promote the proliferation of the transformed prostatic epithelial cells via paracrine and autocrine mechanisms. BioVision’s FGF6 (Human) ELISA Kit is used for the quantitative detection of FGF6 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FGF6 in the sample.


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