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FGF10 (Human) ELISA Kit

A highly sensitive sandwich ELISA kit for the quantitative measurement of FGF10

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5137
$750.00

Product Details

Cat # +Size E5137-100
Size 96 assays
Detection Method Absorbance is measured at 450 nm
Species Reactivity Human
Applications The ELISA kit is used for the quantitative detection of FGF10 in serum, plasma, tissue homogenates and other biological fluids
Features & Benefits ● Detection range: 7.813-500 pg/ml
● Sensitivity: 4.688 pg/ml
● Highly sensitive and specific
● Recovery Rate: 90-104% for serum, 85-104% for EDTA plasma and 87-97% for heparin plasma
● Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10%
Kit Components ● ELISA Microplate
● Wash Buffer (25X)
● Plate Sealers
● Lyophilized Standard (500 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody (Concentrated)
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

FGF10 also known as Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which are glycosaminoglycan binding proteins which possess broad mitogenic and cell survival activities. It plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. FGF family members possess broad mitogenic and cell survival activities. FGF10 is a paracrine signaling molecule seen first in the limb bud and organogenesis development. FGF10 starts the developing of limbs and it is involved in the branching of morphogenesis in multiple organs such as the lungs, skin, ear and salivary glands. It also plays a role in wound healing. FGF10 exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7.FGF10 promotes adult cardiomyocyte cell cycle reentry and directs stem cell differentiation and cell reprogramming toward the cardiogenic lineage provide new insights into therapeutical strategies for cardiac regeneration and repair. FGF10 also maintains progenitor cell populations in the airway and promotes alveolar type 2 cell expansion and differentiation following injury. BioVision’s FGF10 (Human) ELISA Kit is used for the quantitative detection of FGF10 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FGF10 in the sample.


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