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FGF-21 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of FGF-21 in human serum, plasma, tissue lysates, and other biological fluids

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: E5011
$750.00

Product Details

Cat # +Size E5011-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit for quantitative measurement of FGF-21 in serum, plasma, cell and tissue lysates, and other biological fluids
Features & Benefits ● Recovery range: 85 - 105% for normal human serum and plasma samples
● Detection range: 31.25 – 2000 pg/ml
● Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%
● This Sandwich ELISA is highly sensitive and highly specific for the detection of FGF-21 in human samples. There is no significant cross-reactivity or interference between FGF-21 and analogues
● Sensitivity: 18.75 pg/ml
Kit Components ● Micro ELISA plate
● Wash Buffer (25X)
● Plate sealers
● Standard (Lyophilized) (2000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Fibroblast growth factor 21 (FGF-21) is a pleiotropic hormone that belongs to the FGF family of proteins. Liver is the primary source of FGF-21; however, it may also be present in other tissues such as the skeletal muscle, white adipose and brown adipose tissue, and pancreas. FGF-21 expression is regulated by PPARα in the liver, whereas in the adipose tissue, the expression is regulated by PPARγ. FGF-21 is a master regulator of energy homeostasis. Under fasting conditions or ketogenic dietary intake, FGF-21 expression increases and it promotes hepatic gluconeogenesis to produce glucose to other tissues. It stimulates the uptake of glucose only in the adipose tissue but not any other cells. Overexpression of FGF-21 in transgenic mice protects mice from diet-induced obesity and lowers glucose and triglyceride levels in the blood of diabetic rodents. Increased levels of FGF-21 were reported in the serum of patients suffering from Type II diabetes and non-alcoholic fatty liver disease (NAFLD). Thus FGF-21 could serve as an efficient therapeutic target for the treatment of metabolic diseases. BioVision’s FGF-21 (Human) ELISA kit quantitatively measures FGF-21 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of FGF-21 in the sample or standard.


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