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Product Details
| Alternate Name | FGF2, HBGF-2, Prostatropin, Basic fibroblast growth factor, Heparin-binding growth factor 2 |
|---|---|
| Gene Symbol | FGF-2 |
| Gene ID | 2247 |
| Accession # | P09038 |
| Source | E. coli |
| Appearance | Recombinant FGF-2 is available as a lyophilized powder |
| Physical Form Description | FGF-2 protein is lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 + 150 mM NaCl |
| Molecular Weight | Human FGF-2 is a 17.2 kDa recombinant protein |
| Purity by SDS-PAGE | ≥95% |
| Purity by | ≥95% |
| Endotoxin Level | <0.1 ng/μg |
| Biological Activity | The ED₅₀ of human FGF-2 is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 3 ng/ml. |
| Reconstitution Instructions | Reconstitute human FGF-2 in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not to be used in humans |
Details
FGF-2 (FGF2, FGF 2, FGF-basic, basic FGF, bFGF) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-2 protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different FGF-2 isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors.
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