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Fatty Acid Amide Hydrolase (FAAH) Activity Assay Kit (Fluorometric)

Highly Sensitive, minimum sample preparation required
Catalog #: K434

In stock

$455.00

Product Details

Cat # +Size K434-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em= 360/465 nm)
• Applications- Measurement of FAAH activity in animal tissues and cell culture
Detection Method Fluorescence (Ex/Em= 360/465 nm)
Species Reactivity Eukaryotes
Applications Measurement of FAAH activity in biological samples (cell lysate/tissue lysate)
Features & Benefits • Simple, rapid & convenient assay to measure FAAH
• Includes Positive Control (FAAH)
Kit Components • FAAH Assay Buffer
• FAAH Substrate (in DMSO)
• FAAH Positive Control
• AMC Standard (1 mM)
• FAAH Inhibitor (in DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Fatty Acid Amide Hydrolase (FAAH; EC: 3.5.1.99, Oleamide Hydrolase, Anandamide Amidohydrolase) is a mammalian integral membrane enzyme, and is a key regulator in lipid signaling. FAAH degrades naturally occurring fatty acid amides such as cannabinoid anandamide and oleamide, a sleep-inducing substance. In general, fatty acid amides are endogenous lipid ligands which activate the cannabinoid (CB) G-protein coupled receptors CB1 and CB2. CB1 and CB2 modulate physiological and behavioral processes such as pain, and anti-inflammation. Therefore, FAAH is crucial in the termination of fatty acid amide bioactive functions. Recent studies showed that blockage of FAAH activity led to increased fatty acid amides levels in nervous system and peripheral tissues. Thus, the study of FAAH and its potential inhibitors could develop novel treatment strategies for pain and central nerve system disorders due to the analgesic, anxiolytic and anti-inflammatory properties that are observed when elevated fatty acid amides are present in humans. In BioVision’s FAAH Assay Kit, FAAH hydrolyzes a non-fluorescent substrate releasing 7-amino-4-methylcoumarin (AMC), a fluorophore, which can be easily measured at Ex/Em= 360/465 nm. The kit provides a specific inhibitor that can be used to compensate for potential non-specfic background in unknown samples. The stable fluorescence signal is positively correlated to FAAH enzymatic activity in samples. The kit offers a rapid, simple, sensitive, reproducible assay and is suitable for detecting FAAH activity as low as 0.1 µU


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