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FADS2 (Human) ELISA Kit

A Sandwich ELISA kit for quantitative measurement of FADS2 in human serum, plasma, tissue lysates, and other biological fluids.

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4954
$695.00

Product Details

Cat # +Size E4954-100
Size 96 assays
Kit Summary Fatty acid desaturase 2 (FADS2) is a delta-6 fatty acyl CoA desaturase that is involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA). This endoplasmic reticulum enzyme is responsible for the conversion of diet-derived linoleic acid and α-linoleic acid into γ-linoleic and stearidonic acid. Subsequently, in the LC-PUFA biosynthesis pathway, it also desaturates tetracosapentaenoate to tetracosahexaenoate, which is then converted to docosahexanoate, an important lipid required for the growth and development of the brain of infants as well as maintaining normal brain function in adults. Studies have demonstrated abnormal expression of FADS2 in cancers such as breast, liver, and lung, leukemia, and melanoma. FADS2 expression correlates with cell proliferation and migration, tumor growth, and angiogenesis. Hence FADS2 could be a promising target for anticancer therapy. BioVision’s FADS2 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of FADS2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of FADS2 in the sample or standard.
Detection Method Absorbance (450 nm)
Species Reactivity Human
Applications Sandwich ELISA kit to quantitatively measure FADS2 in human serum, plasma, tissue lysate and other biological fluids
Features & Benefits ● Detection range: 78.125 – 5000 pg/ml
● This Sandwich ELISA is highly sensitive and highly specific for the detection of FADS2 in human samples. There is no significant cross-reactivity or interference between FADS2 and analogues
● Recovery range: 85 - 105% for normal human serum and plasma samples
● Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%
● Sensitivity: 46.875 pg/ml
Kit Components ● Micro ELISA plate Wash Buffer (25X)
● Plate Sealers
● Standard (Lyophilized) (5000 pg)
● Sample/Standard Dilution Buffer
● Biotin-labeled Antibody
● Antibody Dilution Buffer
● HRP-Streptavidin Conjugate (SABC)
● SABC Dilution Buffer
● TMB Substrate Solution
● Stop Solution
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Fatty acid desaturase 2 (FADS2) is a delta-6 fatty acyl CoA desaturase that is involved in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA). This endoplasmic reticulum enzyme is responsible for the conversion of diet-derived linoleic acid and α-linoleic acid into γ-linoleic and stearidonic acid. Subsequently, in the LC-PUFA biosynthesis pathway, it also desaturates tetracosapentaenoate to tetracosahexaenoate, which is then converted to docosahexanoate, an important lipid required for the growth and development of the brain of infants as well as maintaining normal brain function in adults. Studies have demonstrated abnormal expression of FADS2 in cancers such as breast, liver, and lung, leukemia, and melanoma. FADS2 expression correlates with cell proliferation and migration, tumor growth, and angiogenesis. Hence FADS2 could be a promising target for anticancer therapy. BioVision’s FADS2 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of FADS2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of FADS2 in the sample or standard.


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