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Factor VIIIa Activity Assay Kit (Fluorometric)

Catalog #: K358

Product Details

Cat # +Size K358-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 360/450 nm)
Applications Determine activity of pure FVIIIa
Detect activity of FVIIIa in plasma, and serum
Features & Benefits • Convenient, and rapid
• The assay can detect activity as low as 1 ng of FVIIIa in a variety of samples.
Kit Components • FVIIIa Assay Buffer
• FXa Substrate-AMC
• Enzyme Mix I
• Enzyme Mix II
• Phospholipids
• FVIIIa Enzyme Standard (2.6 µg)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


The coagulation Factor VIII, also known as anti-hemophilic factor (AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa). BioVision’s Factor VIIIa Activity Assay kit is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader. The assay is simple, rapid and can detect activity as low as 1 ng of FVIIIa in a variety of samples.

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Are the FIXa and FX enzymes of human nature or animal (i.e. mouse rat etc.)?
Factor Xa is isolated from human plasma is te activated form.Factor IX a is from bovine sources.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
 BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.