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Product Details
| Cat # +Size | K305-1000 |
|---|---|
| Size | 1000 assays |
| Detection Method | Fluorescence (Ex/Em = 485/530 nm) |
| Applications | Measurement of cell proliferation in response to growth factors, cytokines, mitogens and nutrients Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals Assessment of physiological mediators and antibodies that affect cell growth. |
| Features & Benefits | • Convenient & non-radioactive • High-throughput • Highly-sensitive: detect as low as 50 viable cells in less than 30 min. • Suitable for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis |
| Kit Components | • Calcein AM • Calcein Dilution Buffer |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Quantification of number of viable cells is an indispensible tool in Cell Biology research. BioVision’s Calcein AM Cell Viability Assay Kit is a fluorometric method for extremely sensitive quantification of viable cells that can detect as low as 50 viable cells in less than 30 min. Calcein AM is a non-fluorescent, hydrophobic compound that easily penetrates intact and live cells. Hydrolysis of Calcein AM by intracellular esterase produces a hydrophilic, strongly fluorescent compound that is retained in the cell cytoplasm and can be measured at Ex/Em = 485/530 nm. The measured fluorescence intensity is proportional to the number of viable cells. This assay kit provides an easy-to-use, non-radioactive, and high-throughput method for cell proliferation, cell viability, chemotaxis, cytotoxicity and apoptosis.
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Can you advise how long will the fluorescent signal (produced from Calcein AM) be retained
in the cell cytoplasm in general?
It is not recommended to wait before measuring the fluorescent signal. The end user should measure the
fluorescence immediately after the 10 min incubation period.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
Md Imam Uddin. A novel method for visualizing and tracking endogenous mRNA in a specific cell
population in pathological neovascularization. Sci Rep. Jan 2021. 33510218.
For more citations of this product click here
