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Product Details
| Alternate Name | Protease K, Endopeptidase K, Tritirachium alkaline proteinase |
|---|---|
| Gene ID | 5621 |
| Accession # | P06873 |
| Source | Tritirachium album |
| Appearance | Liquid |
| Molecular Weight | 28.9 kDa |
| Purity by SDS-PAGE | ≥ 99% (Native PAGE and SDS PAGE assay) |
| Formulation | Recombinant Proteinase K is dissolved in 10mM Tris-HCl (pH 7.5), 1 mM calcium choride and 50% glycerol and provided at a concentration of 20 mg/ml. |
| Specific Activity | ≥ 30 units/mg |
| Handling | Centrifuge the vial prior to opening. |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It is useful for a variety of applications such as isolation of DNA and RNA, preparation of chromosomal DNA, etc. It retains its activity in the presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
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