EZSolution™ DAF-2

A nitric oxide (NO) probe
Catalog #: 2752 | abID: ab146630

Product Details

abID ab146630
Alternate Name 4,5-Diaminofluorescein; 2-​(3,​6-​dihydroxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid
Appearance Liquid
Formulation In DMSO (5 mM)
CAS # 205391-01-1
Molecular Formula C₂₀H₁₄N₂O₅
Molecular Weight 362.34
Purity ≥95% by HPLC
SMILES C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
InChi InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
PubChem CID 10666340
MDL Number MFCD01866182
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


DAF-2 is a highly sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.

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