Product Details
| abID | ab146630 |
|---|---|
| Alternate Name | 4,5-Diaminofluorescein; 2-(3,6-dihydroxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid |
| Appearance | Liquid |
| Formulation | In DMSO (5 mM) |
| CAS # | 205391-01-1 |
| Molecular Formula | C₂₀H₁₄N₂O₅ |
| Molecular Weight | 362.34 |
| Purity | ≥95% by HPLC |
| SMILES | C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O |
| InChi | InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2 |
| InChi Key | LTYUPYUWXRTNFQ-UHFFFAOYSA-N |
| PubChem CID | 10666340 |
| MDL Number | MFCD01866182 |
| Handling | Protect from air and light |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
DAF-2 is a highly sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively. At neutral pH the detection limit for NO is 2-5 nM. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.
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