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Product Details
| Alternate Name | 4,5-Diaminofluorescein diacetate; 2-(3,6-diacetyloxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid; DAF-2 DA |
|---|---|
| Appearance | Liquid |
| Formulation | In DMSO (5 mM) |
| CAS # | 205391-02-2 |
| Molecular Formula | C₂₄H₁₈N₂O₇ |
| Molecular Weight | 446.41 |
| Purity | ≥98% by HPLC |
| SMILES | CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N |
| InChi | InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3 |
| InChi Key | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| PubChem CID | 6603693 |
| MDL Number | MFCD05865101 |
| Handling | Protect from air and light |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
DAF-2 diacetate is a cell-permeable derivative of DAF-2 (Cat. No. 2752) and a very useful indicator of nitric oxide (NO). Upon entry into the cell, DAF-2 diacetate is converted into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.
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