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EZSolution™ DAF-2 diacetate

A nitric oxide (NO) probe
Catalog #: 2753
SKU-Size Size Price Qty
2753-100 100 µg
2753-500 500 µg
2753-1000 1 mg
More Sizes Get Quote

Product Details

Alternate Name 4,5-Diaminofluorescein diacetate; 2-​(3,​6-​diacetyloxy-​4,​5-​diamino-​9H-​xanthen-​9-​yl)-​benzoic acid; DAF-2 DA
Appearance Liquid
Formulation In DMSO (5 mM)
CAS # 205391-02-2
Molecular Formula C₂₄H₁₈N₂O₇
Molecular Weight 446.41
Purity ≥98% by HPLC
SMILES CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N
InChi InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
PubChem CID 6603693
MDL Number MFCD05865101
Handling Protect from air and light
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


DAF-2 diacetate is a cell-permeable derivative of DAF-2 (Cat. No. 2752) and a very useful indicator of nitric oxide (NO). Upon entry into the cell, DAF-2 diacetate is converted into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Can be used for the fluorimetric detection of nitric oxide and in fluorescence microscopy to measure real-time changes in nitric oxide levels in vivo.

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