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EZScreen™ NAD+/NADH Colorimetric Assay Kit (384-well)

based on 3 citations in multiple journalsEZScreen™ NAD+/NADH Colorimetric Assay Kit (384-well)34.1 4
Highly Sensitive, minimum sample preparation required
Catalog #: K958

Product Details

Cat # +Size K958-400
Size 400 assays
Detection Method Absorbance (450 nm)
Species Reactivity Mammalian
Applications BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio
Features & Benefits • Simple procedure; takes ~2 hours
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.
Kit Components • NADH/NAD Extraction Buffer
• NAD Cycling Buffer
• NAD Cycling Enzyme Mix
• NADH Developer
• Stop Solution
• NADH Standard
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Nicotinamide Adenine Dinucleotide (NAD) is a co-enzyme indispensible for the activity of multiple catalytic proteins. NAD+ and NADH, its reduced form, participate in many biological redox reactions. NAD+ is mostly associated with redox enzymes required for catabolic reaction and energy production. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for a sensitive detection of NADH, NAD and their ratio. There is no requirement to purify NADH or NAD from samples. The NADH selective developer develops color while reacting with NADH and can be detected at OD 450 nm. The total NAD can be easily quantified by comparing with the provided NADH standard. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit can detect as low as 0.04 pmol of NADH in a 384 well assay plate. The reaction specifically detects NADH and NAD, but not NADP nor NADPH.

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Eunyoung Ha, Duodenal-Jejunal Bypass Surgery Stimulates the Expressions of Hepatic Sirtuin1 and 3 and Hypothalamic Sirtuin1. J Obes Metab Syndr., Dec 2018;  31089570.
Ana I. Duarte, et al., (2018) Dual Therapy with Liraglutide and Ghrelin Promotes Brain and Peripheral Energy Metabolism in the R6/2 Mouse Model of Huntington’s Disease, Scientific Reports, Jun. 2018, 29895889
Luca Peruzzotti-Jametti, et al., (2018) Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation., Cell Stem Cell, Mar. 2018, 29478844
Sheeran, Freya L. et al. (2016) Posttranslational modifications and dysfunction of mitochondrial enzymes in human heart failure. Am J Physiol Endocrinol Metab. 2016 Aug 1;311(2):E449-60.
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