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EZScreen™ Lactate Dehydrogenase Activity Colorimetric Assay Kit (384-well)

Highly Sensitive, no sample preparation required
Catalog #: K953
$450.00

Product Details

Cat # +Size K953-400
Size 400 assays
Detection Method Absorbance (500 nm)
Species Reactivity Mammalians
Applications • Measure triglycerides in biological samples
• Screen and characterize compounds that influence triglyceride metabolism
Features & Benefits Sensitive assays for measuring LDH activity in various biological samples. The kit has been optimized for high throughput analyses
Kit Components • LDH Assay Buffer
• LDH Substrate Mix (lyophilized)
• NADH Standard (lyophilized)
• LDH Positive Control (lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) present in a wide variety of organisms. LDH catalyzes the interchange of lactate to pyruvate, with the concomitant interconversion of NAD+ to NADH. LDH is present in many organs and tissues including liver, heart, pancreas, kidneys, skeletal muscles, brain, and blood cells. When illness or injury damages cells, LDH may be released into the bloodstream, causing a rise of LDH level in blood. Elevated LDH in serum can indicate a number of unfavorable medical conditions like cerebral attack, heart attack, hepatitis, muscular dystrophy, hemolytic anemia and even certain cancers. Therefore, quantitation of LDH activity has significant clinical importance. BioVision’s EZScreen™ Lactate Dehydrogenase Colorimetric Assay kit, offers a simple and sensitive assay that can detect and quantify the enzymatic activity of LDH. In this assay, LDH oxidizes a substrate utilizing NAD as cofactor. The reduced cofactor (NADH) reacts with a chromophore producing a stable color (OD: 500 nm) that is directly proportional to LDH activity. The assay is suitable to detect the enzymatic activity as low as 0.08 mU in various biological samples


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Chanyang Kim, et al., (2018) IGF-1 protects SH-SY5Y cells against MPP+-induced apoptosis via PI3K/PDK-1/Akt pathway, Endocrine Connections, Apr. 2018, 29459421
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