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Product Details
| Cat # +Size | K951-384 |
|---|---|
| Size | 384 assays |
| Detection Method | Absorbance (590 nm) |
| Species Reactivity | Mammalian |
| Applications | The kit detects 0.5-500 µM lactate samples |
| Features & Benefits | • Simple procedure; takes ~ less than 40 minutes • Fast and convenient |
| Kit Components | • EZScreen™ Lactate Assay Buffer • EZScreen™ Lactate Probe (in DMSO, anhydrous) • EZScreen™ Lactate Enzyme Mix (lyophilized) • EZScreen™ Lactate Standard (100 mM) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Abnormal high concentration of lactate in blood has been related to many important diseases such as diabetes and lactate acidosis. Elevated Lactate concentration in blood is also associated with shock, post cardiac arrest, tissue ischemia, thiamine deficiency, liver disorder, mitochondrial diseases and many more. Therefore detection and quantitation of lactate in from blood is a diagnostic key for several diseases. BioVision’s EZScreen™ Lactate Colorimetric Assay Kit uses an enzyme mix which reacts with L-Lactate specifically. D-Lactate does not react with the enzyme mix. The enzymatic reaction with L-lactate produces a product that generates a color (OD= 590 nm) when reacts with the Lactate probe. The formed product is directly proportional to the amount of L-Lactate present in the sample. The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The kit provides a convenient means for detecting 0.5 to 5 mM Lactate in biological samples such as in blood, blood circulation, in cells, in culture media, in fermentation media etc. in 384 HTP format
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