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EZScreen™ Glycogen Colorimetric Assay Kit (384-Well)

Highly Sensitive, minimum sample preparation required
Catalog #: K960
$550.00

Product Details

Cat # +Size K960-400
Size 400 assays
Detection Method Absorbance (590 nm)
Species Reactivity The assay can detect glycogen as low as 0.025 µg
Applications • Measurement of Glycogen in various tissues/cells
• Analysis of metabolism an cell sginaling in various cells
Features & Benefits • Simple procedure; takes ~ 2 hours
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Glycogen



Kit Components • Hydrolysis Buffer
• Development Buffer
• OxiRed Probe
• Hydrolysis Enzyme Mix
• Development Enzyme Mix
• Glycogen Standard (2.0 mg/ml)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycogen is a branched polysaccharide, made of glucose units that serves as the primary source of energy storage in animals. Most of the glucose residues in glycogen are linked by α-1,4-glycosidic bonds with branching via α-1,6 linkage. In mammals, the two major sites of glycogen storage are liver and skeletal muscle. In liver, glycogen synthesis and degradation are regulated to maintain blood-glucose levels, while stored glycogen in muscle can be processed to meet the energy needs of the muscle itself. Abnormal ability to utilize glycogen is found in patients suffering diabetes and other genetic glycogen storage related diseases. BioVision EZScreen™ Glycogen Colorimetric Assay Kit is an easy and convenient assay to measure glycogen levels in biological samples. In the assay, glucoamylase hydrolyzes the glycogen releasing glucose which is then specifically oxidized to produce a product that reacts with OxiRed Probe to generate color (OD 590 nm). The method is quantitative, rapid, simple, sensitive, and designed for high throughput format. The assay can detect as low as 0.025 µg of glycogen in 384-well assay format.


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