Free Fatty Acid Colorimetric Assay Kit (ab282927)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Sample type: Plasma, Serum, Tissue Homogenate
Overview
-
Product name
Free Fatty Acid Colorimetric Assay Kit
See all Free Fatty Acid kits -
Detection method
Colorimetric -
Sample type
Serum, Plasma, Tissue Homogenate -
Assay type
Quantitative -
Range
0.5 nmol/well - 2.5 nmol/well -
Product overview
This Free Fatty Acid Colorimetric Assay Kit (ab282927) provides a simple method for the quantification free fatty acids. The kit offers a convenient, sensitive, enzyme-based method for the detection of long-chain (C-8 (octanoate) and longer) fatty acids in various biological samples. In the EZScreenTM assay, fatty acids are converted to their CoA derivatives, which are subsequently oxidized by the enzyme mix to yield an intermediate. Formed intermediate then reacts with the Free Fatty acid probe to generate color which can be read by a spectrophotometer at OD 570 nm. Our 384-well format enables analyses of large number of samples on a single plate in a high throughput mode. Sample volumes between 1 and 10 μl can be used with this kit.
Sample types: Serum, plasma, and other body fluids. Animal tissues, Cell culture: adherent or suspension cells, Growth media and foods.
-
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K956 EZScreen™ Free Fatty Acid Colorimetric Assay Kit (384-well). K956-400 is the same size as the 400 test size of ab282927.
Fatty Acids play important roles in normal metabolism and in many disease states. They are precursors to a number of bioactive classes of compounds including prostaglandins and leukotrienes, and have been implicated to play roles in such diverse functions as the immune system, autism, and the inflammatory response. Fatty acid levels are an important measure of the physiologic state. High levels are associated with diabetes mellitus, obesity, endocrine dysfunctions, and metabolic syndrome.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 400 tests ACS Reagent 1 vial Enhancer I 1 x 200µl Enzyme Mix (lyophilized) 1 vial Free Fatty Acid Assay Buffer 1 x 25ml Free Fatty Acid Probe (in DMSO, anhydrous) 1 x 200µl Palmitic Acid Standard 1 x 300µl -
Research areas
-
Relevance
Fatty acids are carboxylic acids with long hydrocarbon chains. The hydrocarbon chain length may vary from 8-30 carbons. Fatty acids play very important roles in normal metabolism and many disease development. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. -
Alternative names
- FFA
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab282927 has not yet been referenced specifically in any publications.