Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

EZScreen™ ATP Colorimetric Assay Kit (384-well)

Highly Sensitive, minimum sample preparation required
Catalog #: K959
$500.00

Product Details

Cat # +Size K959-400
Size 400 assays
Detection Method Absorbance (570 nm)
Species Reactivity All
Applications BioVision’s ATP Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides ATP
Features & Benefits • Simple procedure; takes less than 1 hour
• Fast and convenient
• Kit is designed to be a robust method which enzymatic detection of ATP in numerous biological samples
Kit Components • ATP Assay Buffer
• ATP Probe
• ATP Converter (lyophilized)
• Developer Mix (lyophilized)
• ATP Standard (1 µmol; lyophilized)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Adenosine Triphosphate (ATP) is a derivative of adenosine nucleotide that contains a large amount of biochemical energy stored in its high-energy phosphate bonds. ATP releases energy when it is hydrolyzed into ADP or AMP (Adenosine Di or Mono Phosphate). This energy is used by cells to drive various cellular events like metabolic processes, enzymatic reactions, motility, cell division and muscle contraction. It is also used as a substrate in signal transduction, by kinases to phosphorylate proteins and lipids, and by adenylate cyclase to produce cyclic AMP. In eukaryotic cells, ATP is produced via cellular respiration in mitochondria and by photosynthesis in chloroplasts. Anaerobic bacteria can produce ATP by breaking down nutrients into simpler metabolites, and then using the released energy to form ATP from ADP and Pi (inorganic phosphate). Aerobic bacteria also make ATP from ADP and Pi by ATPase which takes the advantage of ‘proton motive force’ when protons travel back to cytosol from periplasmic space. There are many commercially available kits that detects ATP in femtomoles or less by using luminescence but these products require specialized instrumentation and utilize luciferase which can be difficult to maintain in active form. BioVision’s EZScreen™ ATP Colorimetric Assay Kit is designed to be a robust, simple, stable, colorimetric method which utilizes a series of enzymatic reactions to form a product that is easily quantified at OD 570 nm. The method is rapid, simple, sensitive and designed for high-throughput format using a 384-well plate. The kit can detect as low as 80 pmol of ATP in a 384 well assay plate.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.