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EZScreen™ Alanine Aminotransferase (ALT or SGPT) Activity Assay Kit (384-Well)

Sensitive Assay, HTS
Catalog #: K941

In stock


Product Details

Cat # +Size K941-400
Size 400 assays
Kit Summary • Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Species reactivity- Mammalian
• Applications- The kit detects ALT with a detection limit of 0.25 to 1.25 mU per well.
Detection Method Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species Reactivity Eukaryotes
Applications Evaluation of pentose phosphate pathway
Features & Benefits • Simple procedure
• Fast and convenient
• Sensitive assays for measuring ALT activity in various biological samples and the kit is also suitable for high throughput analyses.
Kit Components • ALT Assay Buffer
• OxiRed Probe
• ALT Enzyme Mix
• ALT Substrate
• Pyruvate Standard
• ALT positive control
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Alanine transaminase (ALT) is a transaminase enzyme (EC It is also called serum glutamic pyruvic transaminase (SGPT) or alanine aminotransferase (ALAT). ALT is found in serum and in various bodily tissues, but is usually associated with the liver. It catalyzes the reaction:
α-ketoglutarate + alanine ⇌ glutamate + pyruvate
It is commonly measured clinically as a part of a diagnostic liver function test to determine liver health. Diagnostically, it is almost always measured in units/liter (U/L). In BioVision’s ALT Assay Kit, ALT catalyzes the transfer of an amino group from alanine to ⍺-ketoglutarate, the products of this reversible transamination reaction being pyruvate and glutamate. The pyruvate is detected in a reaction that concomitantly converts a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can be run in either colorimetric or fluorometric detection mode and can detect a minimum of 0.25 to 1.25 mU of ALT activity per well, using a minimal sample volume as low as0.5 μl.

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