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EZCyp™ Active Human Cytochrome P450 3A4

Unique, cost-effective CYP3A4 catalytic system with active recombinant human CYP3A4 coexpressed with the active NADPH reductase in a semipermeable membrane system.
Catalog #: 7872
SKU-Size Size Price Qty
7872-10 10 mg
$135.00
7872-50 50 mg
$485.00
More Sizes Get Quote

Product Details

Alternate Name Cytochrome P450 3A, Cytochrome P450 3A4, Cyt3A4, CYPIIIA, CYPIIIA4, CYP3A, CYP3A4, Microsomal cytochrome P450 3A4, active CYP3A, human cyp3a, human CYP3A4, recombinant human CYP3A4
Gene Symbol CYP3A4
Gene ID 1576
Accession # P08684
Source Pichia pastoris
Appearance Dry powder
Physical Form Description Lyophilized powder
Molecular Weight 55 KDa
Purity by NA
Endotoxin Level NA
Biological Activity NA
Reconstitution Instructions 100 mM Potassium Phosphate buffer, pH 7.7
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

Cytochrome P450 3A4 (CYP3A4, EC 1.14.13.157) is a member of the cytochrome P450 monooxidase (CYP) family of microsomal xenobiotic metabolism enzymes. CYPs are membrane-bound hemeproteins responsible for Phase I biotransformation reactions, in which lipophilic drugs and other xenobiotic compounds are transformed to more hydrophilic products to facilitate excretion from the body. CYP3A4 is expressed in high levels in the liver and intestines, where it catalyzes oxidation of an extraordinarily wide variety of structurally distinct ligands. More than half of all small molecule drugs commonly used by humans are metabolized by CYP3A4. BioVision’s EZCyp™ 3A4 is a permeabilized and stabilized dried yeast powder preparation containing recombinant human CYP 3A4 and recombinant human P450 NADPH oxidoreductase co expressed in the same preparation.


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My protein is not dissolving in 100 mM Potassium Phosphate buffer, pH 7.7. Could you suggest any other buffer or recommend a protocol to reconstitute?
7872 is a powder that will not dissolve completely. It is used as a suspension. After the reaction is performed, the tube can be centrifuged and the supernatant will contain the metabolite. Potassium Phosphate buffer is suitable for resuspension.
Do you know the P450 content (pmol P450/mg dried protein) of this protein is?
Unfortunately, it is very difficult to assess the P450 content of 7872-10 due to the structure of the product. P450 enzymes are membrane bound and are expressed in yeast cells that are then permeabilized and killed to make yeast “ghosts” that allow substrates and products to enter and exit but keeps the cellular membranes intact. While there are advantages to this system, we have not been able to get a good reading for P450 content.