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EZCut™ SUMO Protease 1 (His-tagged), Yeast Recombinant

>90% Pure, Active, Yeast Recombinant, His-Tagged SUMO Protease 1, to cleave the SUMO tag from recombinant proteins.
Catalog #: 7868
SKU-Size Size Price Qty
7868-500 500 Units
$210.00
7868-2500 2500 Units
$550.00
7868-10000 10000 Units
$2,200.00
More Sizes Get Quote

Product Details

Alternate Name Ulp1 peptidase, SUMO Protease
Gene Symbol ULP1
Gene ID 856087
Accession # Q02724
Source E. coli
Appearance Liquid
Physical Form Description 0.1 mg/ml (100 U/µl) solution in 50 mM Tris-HCl, 100 mM NaCl, 5 mM DTT and 20% glycerol pH 8.0
Molecular Weight 28.7 kDa (403-621 aa + N-terminal Poly-His tag).
Purity by SDS-PAGE ≥90%
Biological Activity The EZCut™ SUMO Protease 1 (His-tagged) has an activity of ≥ 1 X 106 units/mg.
Amino Acid Sequence 403-621 aa + N-terminal Poly-His tag
Handling Centrifuge the vial prior to opening.
Storage Conditions -80°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not to be used in humans

Details

SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, Ubl-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. It is highly specific as it recognizes the tertiary structure of the ubiquitin-like (UBL) protein, SUMO (Smt3), rather than its amino acid sequence. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance functional protein production in prokaryotic and eukaryotic expression systems with significantly improved protein stability and solubility. BioVision’s Recombinant EZCut™ SUMO Protease 1 containing an N-terminal His-tag is expressed in E. coli and purified by proprietary chromatographic techniques. The EZCut™ SUMO Protease 1 can be used to cleave SUMO protein tag from recombinant SUMO-fusion proteins. The optimal temperature for cleavage is 30°C; however, the enzyme is active over wide ranges of temperature and pH. After the completion of the cleavage reaction, the protease can be easily removed from the reaction by affinity chromatography using the Ni chelating resin (Cat. # 6562-1, 10, 100, 500).


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