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EZClick™ Stearoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

for labeling and detection of Stearoylated proteins

WARNING: This product can expose you to chemicals including Formaldehyde, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: K418
$425.00

Product Details

Cat # +Size K418-100
Size 100 assays
Kit Summary BioVision offers EZClick™ Stearoylated Protein Assay Kit (Red Fluorescence) a highly specific, simple and robust method for labeling and detection of Stearoylated proteins.
Detection Method Fluorescence Microscope Imaging and FACS analysis
Species Reactivity Mammalian
Applications • Detection and quantification of biosynthesis, dynamics and turnover of stearic acid modified proteins
• Screening for genotoxic compounds and effectors of protein modifications
• Identification and localization of stearic acid modified proteins
Features & Benefits • Simple procedure
• Highly specific
• Robust method for labeling and detection of Stearoylated proteins
Kit Components • EZClick™ Wash Buffer (10X)
• Copper Reagent (100X)
• Permeabilization Buffer (10X)
• EZClick™ Total DNA Stain (1000X)
• Fixative Solution
• Reducing Agent (20X)
• EZClick™ Stearic Acid Label (1000X, EZClick™ Fluorescent Azide (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Stearoylation is one of the numerous types of post-translational modification in proteins. Stearoylated proteins are involved in the regulation of cellular processes such as apoptosis, growth and metabolism. In this modification Stearic acid, a saturated fatty acid is covalently bound to a protein through the action of long-chain-fatty-acid-[acyl-carrier-protein] ligase. In the presence of ATP, ligase then transfers a Stearate moiety to the target protein forming a carbon-sulfur bond (C=S). Recent studies showed a possible correlation between mitochondrial dysfunction and some diseases including neurodegenerative disease, cancer and aging. Furthermore, it is known that stearoylated Transferrin Receptor (TfR1) inhibits JNK activation thereby reducing mitofusin ubiquitination and restoring mitochondrial function. Studying stearoylation of proteins can shed a light on disease etiology and prevention. BioVision offers EZClick™ Stearoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of Stearoylated proteins. The kit uses Alkynyl Stearic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Stearoylated proteins or detection by FACS for quantitative studies. Labeled stearoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.


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