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EZClick™ Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

WARNING: This product can expose you to chemicals including Formaldehyde, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: K441
$425.00

Product Details

Cat # +Size K441-100
Size 100 assays
Kit Summary • Detection method- Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy
• Sample type- Adherent and suspension cells
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and accurate procedure to measure glycoproteins (Sialic Acid-Modified Proteins) in biological samples.
Detection Method Flow Cytometry (Ex/Em 488/(530/590) nm) and Fluorescence Microscopy
Species Reactivity Mammalian
Applications This assay provides a convenient and accurate procedure to measure glycoproteins (Sialic Acid-Modified Proteins) in biological samples
Features & Benefits Simple, fast, does not require lengthy incubation times
Kit Components • EZClick™ Wash Buffer (10X)
• Fixative Solution
• Permeabilization Buffer (10X)
• EZClick™ ManAz Label (1000X)
• Copper Reagent (100X)
• EZClick™ Fluorescent Alkyne (100X)
• Reducing Agent (20X)
• EZClick™ Total DNA Stain (1000X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Glycans are vital components of glycoproteins, glycolipids, and proteoglycans in all domains of life. Glycosylation occurs co- or post-translationally on >50% of eukaryotic proteins resulting in membrane-associated, intracellular, or secreted glycoproteins that are crucial in cellular processes, protein bioactivity and metabolic turnover. Intracellular glycans mediate protein folding, stability, and trafficking while at the cell surface, they participate in recognition, cell-cell interactions involved in adhesion, migration, and embryonic development; host-pathogen interactions critical for bacterial and viral infections; and initiation of immune response. Aberrant glycosylation profiles correlate with inflammation and are universal feature of cancer, with sialic acids playing an especially prominent role as tumor associated carbohydrate antigens (TACAs). Altered sialylation of tumor cell surfaces is associated with several critical malignant properties that include invasiveness and metastatic potential suggesting its implication in clinical diagnosis. Since glycoproteins are not directly encoded in the genome, methods of characterization and analyses of glycoproteins are of great interest. Thus BioVision offers EZClick™ Sialic Acid (ManAz) Modified Glycoprotein Assay Kit, a highly specific, simple and robust method for labeling and detection of N-linked glycosylation of cell surface proteins. We use a modified mannosamine precursor that is fed directly into the cells, converted to sialic acid by the sialic acid biosynthetic machinery, and transported to the Golgi apparatus for glycan elaboration. Followed by click reaction with alkyne-containing dye, this system offers a powerful method for imaging the localization, trafficking, and dynamics of glycans, or detection by FACS for quantitative studies. Labeled Glycoproteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-alkyne or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.


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