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EZClick™ Palmitoylated Protein Assay Kit (FACS/Microscopy), Red Fluorescence

for labeling and detection of Palmitoylated proteins
Catalog #: K416

Product Details

Cat # +Size K416-100
Size 100 assays
Kit Summary BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins.
Detection Method Fluorescence Microscope Imaging and FACS analysis
Species Reactivity Mammalian
Applications • Identification and localization of palmitic acid modified proteins
• Screening for genotoxic compounds and effectors of protein modifications
• Detection and quantification of biosynthesis, dynamics and turnover of palmytoylated proteins
Features & Benefits • Highly specific
• Simple procedure
• Robust method for labeling and detection of Palmitoylated proteins
Kit Components • EZClick™ Total DNA Stain (1000X)
• EZClick™ Wash Buffer (10X)
• Fixative Solution
• EZClick™ Palmitic Acid Label (1000X)
• Permeabilization Buffer (10X)
• Reducing Agent (20X)
• EZClick™ Fluorescent Azide (100X)
• Copper Reagent (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Palmitoylation occurs when fatty acids, such as Palmitic Acid are covalently attached to the side chains of cysteine (and less frequently to serine, threonine side chains) in proteins. This type of post-translational modification greatly affects cellular trafficking, compartmentalization, and membrane tethering. Palmityltransferase (PAT), the enzyme responsible for this modification, transfers a palmitate moiety from palmitoyl-CoA to the thiol group of cysteine in the target protein. Compared to myristoylation, palmitoylation is reversible and the reverse reaction is catalyzed by thioesterases. The Palmitoylation/Depalmitoylation cycle plays an important role when modified protein shuttles between cellular compartments. PAT mutations are associated with many neurological diseases and cancer progression. BioVision offers EZClick™ Palmitoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of palmitoylated proteins. The kit uses a modified Palmitic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This post translational modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Palmitoylated proteins, or detection by FACS for quantitative studies. Labeled Palmitoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. We provide sufficient materials for 100 assays in a 96-well plate format.

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