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EZClick™ Lipid Peroxidation Assay Kit (FACS/Microscopy), Green Fluorescence

A specific, simple and robust method for labeling and detection of lipid peroxidation.

WARNING: This product can expose you to chemicals including Formaldehyde and Cumene which are known to the State of California to cause cancer. For more information go to
www.P65Warnings.ca.gov.
Catalog #: K2115
$485.00

Product Details

Cat # +Size K2115-100
Size 100 assays
Kit Summary BioVision’s EZClick™ Lipid Peroxidation Assay Kit offers a specific, simple and robust method for labeling and detection of lipid peroxidation.
Detection Method FACS & Microscopy
Applications Screening for potential molecules for induction or inhibition of lipid peroxidation
Features & Benefits This system offers a powerful method for imaging lipid peroxidation in cell membranes and detection by FACS for quantitative studies.
Kit Components ● EZClick™ Wash Buffer (10X)
● Fixative Solution
● Permeabilization Buffer (10X)
● Cumene Hydroperoxide
● EZClick™ Linoleic Acid Label (500X)
● Copper Reagent (100X)
● EZClick™ Fluorescent Azide (100X)
● Reducing Agent (20X)
● EZClick™ Total DNA Stain (1000X)
Storage Conditions -20ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Lipid Peroxidation is a process in which reactive oxygen species (ROS, such as hydroxyl radicals) attack carbon-carbon double bonds present in lipids, particularly lipids containing polyunsaturated fatty acid chains. Lipid peroxidation is a radical chain reaction mechanism consisting of three processes including radical initiation, propagation and termination. In the initiation step, ROS abstract a hydrogen atom from a C=C double bond via one-electron chemistry to form a lipid radical. In the propagation step, the lipid radical reacts with oxygen to generate a lipid peroxyl radical, which can remove the hydrogen from another lipid molecule to generate another lipid radical and continue the chain reaction. In the termination step, enzymatic or chemical antioxidants can quench the lipoperoxyl radical, generating a lipid hydroperoxide, which ultimately decomposes to form a reactive α,β-unsaturated aldehyde. Lipid peroxidation plays an important role in cell biology and human health. Excessive lipid peroxidation in the wake of oxidative stress can lead to cancer and neurological disorders. Research into the mechanisms of lipid peroxidation and screening of inhibitors of oxidative stress may lead to development of the therapeutics for diseases of aging. BioVision’s EZClickTM Lipid Peroxidation Assay Kit offers a specific, simple and robust method for labeling and detection of lipid peroxidation. The kit employs an alkynyl linoleic acid analogue that is incorporated into cellular lipid membranes and is subject to peroxidation by various exogenous ROS or pro-oxidant treatments, followed by a click chemistry labeling reaction with an azide-containing dye. This system offers a powerful method for imaging lipid peroxidation in cell membranes and detection by FACS for quantitative studies.


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