EZClick™ Global RNA Synthesis Assay Kit

Microplate-based assay to detect total RNA content using metabolic labeling techniques
Catalog #: K462 | abID: ab273285

Product Details

abID ab273285
Cat # +Size K462-100
Size 100 assays
Kit Summary • Detection method- Fluorescence (Ex/Em= 494/521 nm)
• Sample type- Adherent and suspension cells
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo
Detection Method Fluorescence (Ex/Em= 494/521 nm)
Species Reactivity Eukaryotes
Applications This assay provides a convenient and accurate procedure to detect and quantify RNA transcription in vitro and in vivo
Features & Benefits Simple, fast, does not require lengthy incubation times
Kit Components • EZClick™ Wash Buffer (10X)
• Fixative Solution
• Permeabilization Buffer (10X)
• EZClick™ RNA Label (1000X)
• Copper Reagent (100X)
• EZClick™ Fluorescent Azide (100X)
• Reducing Agent (20X)
• EZClickTM Total DNA Stain (1000X)
• Actinomycin (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


RNA plays crucial role in coding, decoding and regulation of genes, and protein expression in all living cells. The ability to detect newly synthesized RNA or changes in RNA levels under various physiological conditions, or resulting from disease, environmental damage, or drug treatments is an important aspect of toxicological profiling. Many anti-cancer drugs inhibit transcription, and most transcription inhibitors have useful pharmacological properties. BioVision’s EZClick™ Global RNA Synthesis Assay Kit provides a simple and robust tool for detection of global RNA transcription, temporally and spatially, or changes in RNA levels directly in living cells. De novo synthesized RNA can be detected with a simple procedure without the use of radiolabeling or antibodies. The kit principle relies on the incorporation of cell permeable 5-EU (Ethynyl uridine) into nascent RNA, but not into DNA, instead of its natural uridine analog. 5-EU can be used as a replacement for BrU (5-Bromo-uridine) to measure de novo synthesized RNA in proliferating cells. Modified RNA is detected by click chemistry using an azide-containing dye that enables for multiplex analyses with other probes, or detection of RNA-interactive proteins for deeper biological insights. The kit provides sufficient materials for 100 assays for quantitative analysis by microplate reader (Ex/Em= 494/521 nm) and/or by fluorescence microscope. The kit includes Actinomycin D, an inhibitor RNA synthesis that serves as an experimental control.

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