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EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

Metabolic Labeling of Protein Synthesis
Catalog #: K459

In stock

$425.00

Product Details

Cat # +Size K459-100
Size 100 assays
Kit Summary • Detection method- Flow Cytometry (Ex/Em 488 (440/590) nm) and Fluorescence Microscopy
• Sample type- Adherent and suspension cells
• Species reactivity- Mammalian
• Applications- This assay provides a convenient and accurate procedure to measure de novo Protein synthesized in biological samples.
Detection Method Flow Cytometry (Ex/Em (488)/(440/490) nm) and Fluorescence Microscopy
Species Reactivity Eukaryotes
Applications This assay provides a convenient and accurate procedure to measure de novo protein synthesized in biological samples.
Features & Benefits Simple, fast, does not require lengthy incubation times
Kit Components • EZClick™ Wash Buffer (10X)
• Fixative Solution
• Permeabilization Buffer (10X)
•EZClick™ Protein Label (400X)
• Copper Reagent (100X)
• EZClick™ Fluorescent Azide (100X)
• Reducing Agent (20X)
• EZClick™ Total DNA Stain (1000X)
• Cycloheximide (100X)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cells generate a complete set of proteins during division. Protein synthesis is a tightly regulated process and many critical controls in gene expression occur at the level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc.). Protein synthesis is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical parameters in determining the physiological state of the cell. Methods enabling detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important tools in assessment of cytotoxicity. Biovision’s EZClick™ Protein Synthesis Monitoring Assay Kit utilizes a novel and robust chemical method based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Once inside the cell, OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs, OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. Our kit provides sufficient materials for 100 assays to detect nascent proteins synthesized under various physiological conditions, and Cycloheximide, an inhibitor of protein synthesis that serves as an experimental control.


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