Product Details
| Cat # +Size | K2070-100 |
|---|---|
| Size | 100 assays |
| Kit Summary | BioVision’s EZClick™ EdU Proliferation/DNA Synthesis Kit provides a convenient and accurate procedure to measure de novo DNA synthesized in biological samples. |
| Detection Method | Flow Cytometry (Ex/Em 480/(530/590) nm) and Fluorescence Microscopy |
| Species Reactivity | Mammalian |
| Applications | ● Detection of DNA synthesis in proliferating cells and assessment of the cell cycle phase ● Screening for genotoxic compounds and effectors of cell division cycle ● Evaluating effects of anti-cancer drugs and genotoxic agents |
| Features & Benefits | This assay provides a convenient and accurate procedure to measure de novo DNA synthesized in biological samples. |
| Kit Components | ● EZClick™ Wash Buffer (10X) ● Fixative Solution ● Permeabilization Buffer (10X) ● EZClick™ EdU DNA Label (1000X) ● Copper Reagent (100X) ● EZClick™ Fluorescent Azide ● Reducing Agent (20X) ● EZClick™ Total DNA Stain (1000X) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
All living cells undergo a cell division cycle, a biological process crucial for proliferation and inheritance. Cell-division cycle is a series of events resulting in two daughter cells containing replicas of DNA from the original DNA molecule. DNA replication occurs in the S phase of the cell cycle and involves de novo synthesis of genomic DNA from its precursors. The ability to monitor and obtain detailed characterization of the cell cycle and DNA synthesis in proliferating cells is fundamental in basic, and applied immunologic and oncologic studies. Accurate determination of the effect of biologically active reagents on DNA synthesis and cell cycle is of great importance in anti-cancer drug discovery and basic biology. BioVision’s EZClick™ EdU Proliferation/DNA Synthesis Kit utilizes a novel approach that relies on the incorporation of 5-EdU (5-ethynyl-2’deoxyuridine), a nucleoside analog to thymidine into the newly synthesized DNA directly in the cell culture. Incorporation of EdU into genomic DNA in S-phase is detected based on a click reaction between the alkyne moiety of the EdU and fluorescent azide. Compared to historically used BrdU reactions, click reaction is carried out under mild conditions and flow cytometry/fluorescence microscopy can be used for the assessment of proliferating cells in the population. The kit provides sufficient materials for 100 assays based on the protocol below.
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