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EZCell™ Phagocytosis Assay Kit (Red Zymosan)

Detects Apoptotic DNA within 90 min, no extractions or columns

WARNING: This product can expose you to chemicals including Trypan Blue, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: K398
$350.00

Product Details

Cat # +Size K398-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 540/570 nm)
Species Reactivity Mammalian
Applications Rapid detection, quantification and validation of phagocytosis in convenient 96-well format • Tracking ligand internalization and screening for effectors of phagocytosis
Features & Benefits • Simple & Rapid Protocol
• Convenient: Non-Radioactive, no special handling or disposal required
• High-Throughput
• Accurate: reproducible results with low intra & inter assay variability
Kit Components • Phagocytosis Assay Buffer
• Red Zymosan
• 10X Quenching Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays. BioVision’s EZCell™ Phagocytosis Assay Kit (Green Zymosan) utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. Our kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).


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The Buffer Additive that came with the kit has some precipitation (white fluffy stuff) in it. Is it ok to use it for the experiment?
The Buffer Additive has FBS in it and sometimes it precipitates. The precipitate contains fibrin (clot forming protein, made up from fibrinogen subunits) and lipoproteins. This is a normal characteristic of FBS, especially heat-inactivated FBS, and will not affect the product performance. You can do two things 1) try not to pipette it when you use it for the assay or 2) you can centrifuge the buffer additive vial and simply let them settle to the bottom of the vial and then use the additive for the assay. Either way, the presence of the precipitate is not going to affect the quality of the product.