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EZCell™ Phagocytosis Assay Kit (Green E. coli)

WARNING: This product can expose you to chemicals including Trypan Blue, which is [are] known to the State of California to cause cancer.  For more information go to www.P65Warnings.ca.gov.
Catalog #: K963
$350.00

Product Details

Cat # +Size K963-100
Size 100 assays
Detection Method Fluorescence (Ex/Em = 490/520 nm)
Species Reactivity Mammalian
Applications • Screening for potential inhibitors that disrupt the interaction between p53 and MDM2
Features & Benefits • Simple & Rapid Protocol
• Convenient: Non-Radioactive, no special handling or disposal required
• High-Throughput
• Accurate: reproducible results with low intra & inter assay variability
Kit Components • Phagocytosis Assay Buffer
• Green E. Coli
• 10X Quenching Solution
Storage Conditions 4°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as a pathogen in phagocytosis assays. BioVision’s EZCell™ Phagocytosis Assay Kit (Green E. coli) utilizes heat-killed, fluorescently pre-labeled E. coli particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.


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Michele S. Barros. Aedes aegypti saliva impairs M1-associated proinflammatory phenotype without promoting or affecting M2 polarization of murine macrophages. Parasit Vectors May-19. 31097013.
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