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EZCell™ Intracellular Nitric Oxide Synthase (NOS) Detection Kit

Market's first and only cell based assay with a simple, one step, non-radiometric protocol for detection of intracellular NOS.
Catalog #: K207

In stock

$275.00

Product Details

Cat # +Size K207-100
Size 100 assays
Detection Method Detection method- fluorescence microscope (Ex/Em = 485/530)
Species Reactivity Mammalian cells
Applications Detection of NOS activity in adherent cells.
Screening/studying/characterizing stimulators/inhibitors that affect intracellular levels of NOS.
Features & Benefits • Simple one step staining procedure
Takes only 1-2 hrs
• Non-radiometric fluorescent detection
Kit Components • NOS Assay Buffer
• Staining Dye (In DMSO)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Nitric oxide synthases (EC 1.14.13.39) (NOSs) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved with the regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as a part of body’s defense mechanism. BioVision’s Intracellular Nitric Oxide Synthase Detection kit uses a dye that reacts with intracellular NO produced by NOS to produce fluorescence (Ex/Em = 485/530 nm), which is proportional to the concentration of intracellular NOS and can be detected using a microplate reader or a fluorescence microscope. This kit provides a simple, non-radiometric method for detection of intracellular NOS in the cells.


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Can we use frozen samples with this assay?
This kit can only work with live cells. You can refer to our products K205-100 and K206-100 for frozen samples.
Can detect the NO level of tissues or just the NOS enzymes?
The kit allows you to calculate the NOS enzyme activity in your tissues.
Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
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