Product Details
| abID | ab284552 |
|---|---|
| Cat # +Size | K504-100 |
| Size | 100 assays |
| Detection Method | Fluorescence (Ex/Em = 380/460 nm) |
| Applications | • Rapid and sensitive detection of intracellular levels of GSH and activity of GST in live cells • Screening for compounds affecting intracellular GSH levels |
| Features & Benefits | • Highly sensitive fluorometric method to measure intra-cellular Glutathione levels in response to a variety of biochemical stimuli • Simple & High throughput-adaptable • Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect Glutathione metabolism |
| Kit Components | • Wash Buffer • Monochlorobimane (MCB) |
| Storage Conditions | -20°C |
| Shipping Conditions | Gel Pack |
| USAGE | For Research Use Only! Not For Use in Humans. |
Details
Glutathione (GSH) represents major low molecular-weight free thiol in living cells. As the key antioxidant in mammalian cells, intracellular GSH forms rapidly eliminated conjugates with electrophilic xenobiotics, free radicals as well as hydroperoxides. Insufficiency of cellular GSH results in oxidative stress damage and mitochondrial degeneration. Diminished levels of glutathione are symptomatic of cell stress thus; it is advantageous to monitor distribution of the intracellular GSH as an indicator of early apoptosis and cell death. BioVision’s EZCell™ Glutathione Detection Kit (Blue Fluorescence) utilizes Monochlorobimane (MCB), a non-fluorescent and cell-permeable dye, that forms highly fluorescent adducts with GSH (GSH-MCB) detectable at EX/EM= 380/465 ±20 nm respectively. The reaction is catalyzed by glutathione-S-transferase (GST) and the intensity of the fluorescence reflects the amount of intracellular GSH. Hence, our kit provides a useful tool for fast and easy evaluation of the GSH effectors directly in the living cells.
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BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways. |
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Can the kit work on bacteria or yeast cells?
The kit has been standardized for mammalian cells only
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. You have to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Do you have trial sizes of this kit?
Unfortunately, we do not have trial sizes of this kit available. However, if you are based in the US or Canada, we will give you a 10% off list price introductory discount on its purchase price. If you are based out of this area please contact your regional BioVision distributor.
What is the shelf life of this kit?
BioVisions’s assay kits expire 6 months from the date of shipment. Some components of the kit may expire sooner as mentioned in the data sheet
Can we purchase individual components of this kit?
Yes, you can purchase any of the kit's components without the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
Can we use an alternate buffer for sample preparation (cell lysis, sample dilutions etc)?
Our assay buffers are optimized for the reactions they are designed for. They not only contain some detergents for efficient lysis of your cells/tissue, but also contain some proprietary components required for the further reactions. Therefore, we highly recommend using the buffers provided in the kit for the best results.
