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EZAct™ Procathepsin Activation Buffer

A ready-to-use buffer for generation of active Cathepsins by auto-activation of the Procathepsins.
Catalog #: 1030
$95.00

Product Details

Cat # +Size 1030-1
Size 1 ml
Highlights APPLICATIONS:
1. To generate active Cathepsins from inactive Procathepsins.
2. To test the activity of Cathepsins.
3. To screen for Cathepsin Inhibitor candidates.
ACTIVATION PROTOCOL:
1. Adjust the concentration of the target Procathepsin to 0.5-5 mg/ml with the appropriate buffer.
2. Add equal amounts of EZAct™ Procathepsin Activation Buffer and mix well by pipetting up and down.
3. Incubate at the appropriate temperature (4°/ 22°/ 37°C) for the study.
4. Check the cathepsin activation by testing with an appropriate substrate.
Note: BioVision’s Cathepsin Assay kits can be used for assaying the Cathepsin activity. Choose from assay kits: K140, K141, K142, K143, K144, K145 and K146.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Cathepsins (CTS) are proteases that degrade proteins. They are present in all animals and other organisms. These proteases are of three types: cysteine proteases, aspartyl protease and serine proteases. Cathepsins are produced as inactive proenzyme precursors that are subsequently activated in lysosomes. Biovision’s EZAct™ Procathepsin Activation Buffer is a ready-to-use buffer for generation of active cysteine/aspartyl Cathepsins by auto-activation of the Procathepsins.


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