Express CRISPR sgRNA Synthesis Kit

Express CRISPR sgRNA Synthesis Kit
Catalog #: K1253 | abID:

Product Details

Cat # +Size K1253-25
Size 25 Reactions
Kit Summary A neat handy tool for generating sgRNAs for spCas9 system.
Applications • sgRNA in vitro studies
• sgRNA in vitro screening
Features & Benefits • Easy 1-step setup
• Short reaction time, only 30 mins
• RNaseOFF ribonuclease inhibitor included in the reagents for extra protection
Kit Components • 2X Express sgRNA Synthesis SuperMix
• DNA Polymerase
• sgRNA Control Oligo (10 μM)
• OneScribe Enzyme Mix
• DNase I, RNase-Free (2 U/μl)
• 10X DNase I Reaction Buffer
• Nuclease-free H2O
Storage Conditions -20°C
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.


BioVision’s Express CRISPR sgRNA Synthesis Kit (S. pyogenes) is a neat handy tool for generating sgRNAs for spCas9 system.

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Is it necessary to use DNAse I for sgRNA synthesis.
DNAse I treatment will remove DNA template, leftover NTPs and dNTPs. A reliable concentration will be useful for downstream applications.