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Erythropoietin (Rat) ELISA Kit

A sandwich ELISA kit for in vitro quantitative determination of rat EPO concentrations in serum, plasma and other biological fluids
Catalog #: E4732
$525.00

Product Details

Cat # +Size E4732-100
Size 100 assays
Detection Method Absorbance (450 nm)
Species Reactivity Rat
Features & Benefits • Specificity: No significant cross-reactivity or interference between Rat EPO and analogues was observed.
• Detection Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL
• Coefficient of variation is < 10%.
Kit Components • Plate Sealer
• HRP Conjugate (100x)
• Biotinylated Detection Ab (100x)
• Stop Solution
• Standard & Sample Diluent
• Micro ELISA Plate
• Wash Buffer (25x)
• Biotinylated Detection Antibody Diluent
• Reference Standard
• HRP Conjugate Diluent
• Substrate Reagent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Erythropoietin is a member of the EPO/TPO family. EPO is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. BioVision's EPO ELISA kit is based on the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to rat EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for rat EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. The substrate solution is added to each well which reacts with rat EPO, biotinylated detection antibody and Avidin-HRP conjugate to give blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of rat EPO. The concentration of rat EPO in the samples can be calculated by comparing the OD of the samples to the standard curve.


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