Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Erythromycin ELISA Kit

A Competitive ELISA kit for quantitative measurement of Erythromycin in Milk, Eggs, Urine, Serum, and Muscle samples

WARNING:
This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California
to cause cancer. For more information go to www.p65warnings.ca.gov/.
Catalog #: E4955
$695.00

Product Details

Cat # +Size E4955-100
Size 96 assays
Detection Method Absorbance (450 nm)
Species Reactivity Universal
Applications Competitive ELISA kit to quantitatively measure Erythromycin in tissue, milk, egg, urine and serum samples
Features & Benefits ● Detection Limit: 2ppb for raw milk and egg, 5ppb for urine, serum and muscle (livestock), 10ppb for muscle (fish and shrimp)
● This ELISA kit is used for in vitro quantitative determination of Erythromycin
● Sensitivity: 0.1ppb
● Cross reaction: Erythromycin 100%, Spiramycin, Valnemulin, Lincomycin, Kanamycin, Tilmicosin, Doramectin, Ivermectin, Eprinomectin, Moxidectin, Abamectin, Tylosin all<0.1%
Kit Components ● Micro ELISA Plate Plate Sealer
● Standard (S0 – S5)
● HRP Conjugate
● Antibody working solution
● Substrate A
● Substrate B
● Stop Solution
● Wash Buffer (20X)
● Tissue diluent
Storage Conditions 4ºC
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Erythromycin is a broad-spectrum antibiotic that belongs to the class of macrolides that contains Azithromycin, Spiramycin, and others. This bacteriostatic drug is produced by Saccharopolyspora erythraea and is effective against both gram-positive and gram-negative bacteria. The mechanism of action is that erythromycin binds to 23S rRNA of the 50S ribosomal subunit. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. Antimicrobials such as Erythromycin are extensively used in food-producing animals to treat infectious diseases. According to International Food Standards, Maximum Residue Limits (MRLs) for erythromycin for tissues of food-producing species (such as chicken and turkey) is 100 μg/kg (ppb) and in eggs is 50 μg/kg (ppb). BioVision’s Erythromycin ELISA kit is used to quantitatively measure Erythromycin in tissue and milk samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Erythromycin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Erythromycin in the samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.