Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

ER Transcription Factor Activity Assay Kit (Colorimetric)

A 96-well plate based colorimetric assay to measure the activation of estrogen receptor (human, mouse and rat) in nuclear extracts or cell lysates.

WARNING: This product can expose you to chemicals including Sulfuric acid and TMB, which are known to the State of California to cause cancer. For more information go to www.P65Warnings.ca.gov.
Catalog #: K2105
$695.00

Product Details

Cat # +Size K2105-100
Size 100 assays
Kit Summary BioVision’s ER Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of ER in nuclear extracts or cell lysates.
Detection Method Absorbance at 450 nm
Species Reactivity Human, Rat, Mouse
Applications Semi-quantitative measurement of activation of estrogen receptor (human, mouse and rat) in nuclear extracts or cell lysates.
Features & Benefits The kit offers an easy, rapid, sensitive and non-radioactive way to measure the activation of estrogen receptor (human, mouse and rat) in nuclear extracts or cell lysates.
Kit Components ● Plate Coated with DNA Probes
● Non-Competitor Oligo
● TMB Substrate
● Stop Solution
● Positive Control
● Plate Sealing Film
● Binding Buffer (5X)
● DTT (100 mM)
● Protease Inhibitor Cocktail
● ER Primary Antibody
● Antibody Diluent Buffer
● HRP Conjugate Stock
● Wash Buffer (10X)
● Competitor Oligo
Storage Conditions Multiple Temperatures
Shipping Conditions Dry Ice
USAGE For Research Use Only! Not For Use in Humans.

Details

Estrogens including estrone, estradiol, estriol, and estretrol belong to the family of organic compounds known as steroids. They are primarily synthesized in the ovaries, but also in the adrenal glands and adipose tissue. They are involved in regulating many physiological processes including reproduction, cardiovascular health, bone integrity, cholesterol mobilization, brain function etc. Estrogen mediates its action by binding to the estrogen receptor (ER) in the nucleus. The receptors then dimerizes and bind to specific response elements known as estrogen response elements (EREs) in the promoters of the target genes. BioVision’s ER Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of ER in nuclear extracts or cell lysates. The kit offers easy, rapid, sensitive and non-radioactive way to detect the activation of transcription factors in samples. In this assay, double stranded DNA sequence containing the ERE is coated on the 96-well plate. Active ER in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of ER primary antibody that recognizes the ER-ERE comlex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.