Call 408.493.1800 | Fax 408.493.1801 | Toll Free 800.891.9699 (US Only) | Email: [email protected]

Enolase Activity Colorimetric/Fluorometric Assay Kit

based on 1 citations in multiple journalsEnolase Activity Colorimetric/Fluorometric Assay Kit14.1 4
Only kit on the market to measure enolase activity
Catalog #: K691

In stock

$635.00

Product Details

Cat # +Size K691-100
Size 100 assays
Detection Method Absorbance (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm)
Applications Measurement of enolase activity in various tissues/cells
Analysis of glycolytic metabolism in different tissues/cells
Mechanistic studies of various cancers
Screening anti-cancer drugs
Features & Benefits • Rapid and easy-to-use.
• Highly-sensitive.
• This assay kit can measure less than 0.04 mU of enolase activity in a variety of samples.
Kit Components • Enolase Assay Buffer
• OxiRed™ Probe (in DMSO)
• Enolase Substrate Mix (Lyophilized)
• Enolase Converter (Lyophilized)
• Enolase Developer (Lyophilized)
• Enolase Positive Control (Lyophilized)
• H2O2 Standard (0.88 M)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

Enolase (EC 4.2.1.11), also called 2-phospho-D-glycerate hydrolase or 2-phosphoglycerate dehydratase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate to phosphoenolpyruvate (PEP) & also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, Enolase consists of three subunits: α, β and γ that combine into 5 isozymes, 3 of which are commonly found in human tissues. This enzyme exists in all organisms, which can undergo glycolysis. Besides its role in glycolysis, enolase has other functions too. Recent studies have shown that increased activity of enolase is associated with tumor invasion and metastasis; therefore, precise measurement of enolase activity may be of great interest for tumor diagnosis and prognosis. In BioVision’s Enolase Activity Assay Kit, enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed™ probe to generate color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This simple & sensitive assay Kit can detect enolase activity less than 0.04 mU in a variety of samples.


Why buy BioVision Products?
Innovation
Affordability
Global Presence
Technical Support
BioVision aims to provide our customers innovative tools for accelerating drug discovery and biological research. BioVision offers >8,000 products including the most comprehensive array of assay kits for key targets in Metabolic pathways.
BioVision is committed to providing the highest quality products at a competitive price.
We have a broad network of global distributors who are ready to address your research needs and ensure fast delivery.
Our highly trained Technical Support team provides comprehensive product support and is dedicated to resolving your issues quickly and efficiently.
In this assay do you measure quantity of hydrogen peroxide created in the reaction process that goes from PEP to the intermediate?
Yes, the principle of this kit is to measure hydrogen peroxide (generated at the last step of the metabolite conversion) by interaction with an OxiRed probe which generates color (OD 570 nm) or fluorescence (Ex/Em = 535/587 nm). This kit can detect Enolase activity < 0.04 mU in a variety of samples.
Can frozen samples be used with this assay?
Fresh samples are always preferred over frozen samples. However, frozen samples can also be used, provided, they were frozen right after isolation, were not freeze thawed multiple time (for which we recommend aliquoting the samples before freezing) and have been frozen for relatively short periods.
Is it possible to use a different wavelength than recommended for the final analysis?
It is always recommended to use the exact recommended wavelength for the most efficient results. However, most plate readers have flexibility in their band width of detection in increments of +/- 10 nm. Depending on this flexibility range, you can deviate from the recommended wavelengths within limits.
What is the exact volume of sample required for this assay?
There is no specific volume we can recommend for the amount any sample to be used since it is completely sample concentration and quality based. It is recommended to do a pilot expt with multiple sample volumes to determine the optimal volume which gives a reading within the linear range of the standard curve. Please refer to the citations for this product to see what other clients have used with similar sample types.
Are trial sizes of this kit available?
Unfortunately, we do not have trial sizes of this kit available. However,for customers based in the US or Canada, a 10% off list price introductory discount can be offered on the list price. For all other customers based out of this area our regional BioVision distributor should be contacted.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Can individual components of this kit be purchased separately?
Yes, any of the kit's components can be purchased separately without having to buy the whole kit. Please refer to the component Cat #s mentioned on the datasheet for ordering.
What is the shelf life of this kit?
This kit is good for 12 months from the date of shipment in the unopened form when stored at the appropriate temperature and appropriate conditions. After opening and reconstitution, some of the components in this kit are good for 2 months at -20°C. Please refer to the datasheet for storage information and shelf life of each of the components.
Cho, Haaglim et al. (2017) ENOblock, a unique small molecule inhibitor of the non-glycolytic functions of enolase, alleviates the symptoms of type 2 diabetes, Sci Rep. 2017 Mar 8;7:44186.
For more citations of this product click here