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Enhanced Green Fluorescent Protein (EGFP)

based on 8 citations in multiple journalsEnhanced Green Fluorescent Protein (EGFP)84.1 4
Fluorescent protein ideal for subcellular labeling/visualization
Catalog #: 4999
SKU-Size Size Price Qty
4999-100 100 μg
$340.00
4999-1000 1 mg
$2,365.00
4999-5000 5 mg
$6,985.00
More Sizes Get Quote

Product Details

Alternate Name E-GFP, Enhanced Green Fluorescent Protein
Source E. coli
Appearance Lyophilized protein
Physical Form Description Freeze Dried
Molecular Weight 32.7 kDa
Purity by SDS-PAGE ≥97%
Purity by ≥97%
Endotoxin Level <0.1 ng/μg
Reconstitution Instructions Reconstitute with PBS to 1 mg/ml
Handling Centrifuge the vial prior to opening.
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not to be used in humans

Details

The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP (Gene Bank Accession no. U57607) is a 32.7 kDa monomer with 293 amino acids tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm (ε489 nm = 55,000 M⁻ˡ Cm⁻ˡ; ε280 nm = 21980 M⁻ˡ Cm⁻ˡ).


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What is the amino acid sequence of your recombinant EGFP?
Cat# 4999 has an amino acid sequence ranging from 2 -238 of Uniprot id P42212 with several mutations and His-tags are present at both N and C-terminus.
King et al., Removing T-cell epitopes with computational protein design. PNAS, Jun 2014; 111: 8577 - 8582.
Kallenberger et al., Intra- and Interdimeric Caspase-8 Self-Cleavage Controls Strength and Timing of CD95-Induced Apoptosis. Sci. Signal., Mar 2014; 7: ra23.
Lawrimore, J. et. al. Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome. J. Cell Biol., Nov 2011; 195: 573 - 582.
Moeller L et al (2009) J. Exp. Bot.; 10.1093/jxb/erp167.
Velazques VM et al (2009) Blood; 113: 538 - 545.
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