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Endothelin Converting Enzyme 1 Activity Assay Kit (Fluorometric)

Industry's first kit to detect ECE1 activity in biological samples

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Catalog #: K536

Product Details

Cat # +Size K536-100
Size 100 assays
Kit Summary • Detection method - Fluorescence (Ex/Em = 320/420 nm)
• Applications- Detection of ECE1 activity in tissue/cell lysates/protein preparations
Detection Method Fluorometric (Ex/Em = 320/420 nm)
Species Reactivity Mammalian
Applications Detection of ECE1 activity in tissue/cell lysates
Features & Benefits • Simple one-step reaction
• Takes only 1-2 hrs
• Non-radiometric fluorescent detection
• HTP adaptable
Kit Components • ECE-1 Assay Buffer
• ECE-1 (lyophilized)
• ECE-1 Substrate (in DMSO)
• ECE-1 Inhibitor Mix
• MCA Standard (5 mM)
Storage Conditions -20°C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.


Endothelin Converting Enzyme 1 (ECE1; EC: is a membrane-bound metalloprotease that cleaves inactive big endothelin (big ET-1), which is the precursor of active endothelin. Studies have shown that big ET-1 could be used as a predictor of survival in patients suffering esophageal squamous cell carcinoma. ECE-1 is abundantly expressed in vivo in endothelial cells producing mature ET-1. As of today, Endothelin is the most potent vasoconstrictor known and it has cytokine- or hormone-like activities. Therefore, ECE-1 could play a significant role in pathogenesis of cardiovascular diseases and Alzheimer’s Disease. BioVision’s Endothelin Converting Enzyme 1 Activity Assay Kit utilizes the ability of active ECE-1 to cleave a synthetic substrate (MCA-based peptide) releasing free fluorophore, in the presence or absence of the ECE-1 Inhibitor Mix. The released f.luorophore can be easily quantified using a fluorescence microplate reader. Our kit uses a unique combination of substrate and inhibitor that specifically detects ECE-1 in a variety of biological samples. Contribution of other enzymes with similar catalytic properties - i.e. Endothelin Converting Enzyme 2 (ECE-2), Angiotensin-Converting Enzyme (ACE1, ACE2), and Neprilysin is compensated for within the assay. Our assay kit is simple, specific and can detect as low as 0.5 µU of ECE-1 activity.

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