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EHMT2 Antibody

Polyclonal antibody to detect EHMT2 in human, mouse and rat samples.
Catalog #: 6152
$335.00

Product Details

Cat # +Size 6152-100
Size 100 μg
Antibody Target EHMT2
Alternate Name BAT8, C6orf30, DKFZp686H08213, FLJ35547, G9A, KMT1C, NG36, NG3
Host Rabbit
Antibody Type Polyclonal
Isotype Rabbit IgG
Immunogen Recombinant protein of human EHMT2
Accession # Q96KQ7
Gene ID 10919
Appearance Colorless liquid
Concentration 1 mg/ml
Formulation 100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
Purification Affinity purified
Species Reactivity Human, mouse, rat
Application Western blot, Immunohistochemistry
Positive Control Western Blot: Various cell lysates
Application & Usage Western blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
Handling The antibody solution should be gently mixed before use.
Storage Conditions -20 °C
Shipping Conditions Gel Pack
USAGE For Research Use Only! Not For Use in Humans.

Details

G9a, also known as Euchromatic histone­lysine N­methyltransferase 2 (EHMT2), is a member of a family of histone lysine methyltransferases. Recombinant G9a can mono­, di­ and tri­methylate histone H3 on Lys9 and Lys27 in vitro. However, in vivo G9a forms a complex with GLP, a G9a­related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono­ and di­methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing. G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero­dimerization and complex stability. Wiz contains two CtBP co­repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co­repressor complex. In addition, G9a and GLP are components of other large transcriptional co­repressor complexes, such as those involving E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication. G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes. In addition, G9a facilitates increased global levels of di­methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocellular carcinoma.


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