Cell Transformation Assay Kit (Colorimetric) (ab235698)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Cell Transformation Assay Kit (Colorimetric)
See all Cell Transformation kits -
Detection method
Colorimetric -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Transformation of normal cells into neoplastic (malignant) cells is the first step in tumorigenesis. In vitro assays of cellular oncotransformation, which measures phenotypic alterations to cultured cells that mirror those observed in tumors, are a critical tool in studying the mechanisms of carcinogenesis. Cell transformation assays are frequently employed in toxicology to evaluate the carcinogenic potential of a particular compound. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. In this assay, transformed cultured cells gain the ability to proliferate through the soft agarose gel without attaching to a surface and tend to form spheroid-like clumps of cells (colonies). However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay Kit (Colorimetric)(ab235698) is faster, stable and more sensitive than the traditional Soft-Agar Assay. The kit is based on the conversion of the tetrazolium salt (WST) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has a wide linear range from 10,000-400,000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K921 Cell Transformation Assay Kit (Colorimetric). K921-100 is the same size as the 100 test size of ab235698.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Agarose Powder 1 x 240mg 10X DMEM Solution 2 x 1.5ml Electro Coupling Solution 1 x 1.8ml Staining Solution 1 x 1ml WST Reagent III 1 vial
Images
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(A) HeLa (human epithelial cell line from cervix adenocarcinoma) cell-dose curve; (B) Equal numbers of NIH/3T3 (mouse embryo fibroblast cell line) and HeLa cells were seeded for the Cell Transformation Assay. After 6 days, the cell numbers were measured. Standard and Samples readings were taken 4 hours after adding WST Working Solution; (C) Image of HeLa cell colonies. HeLa cells were cultured for 6 days according to the kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab235698 has been referenced in 2 publications.
- Canals J et al. Human embryonic mesenchymal lung-conditioned medium promotes differentiation to myofibroblast and loss of stemness phenotype in lung adenocarcinoma cell lines. J Exp Clin Cancer Res 41:37 (2022). PubMed: 35081981
- Laliotis GI et al. AKT3-mediated IWS1 phosphorylation promotes the proliferation of EGFR-mutant lung adenocarcinomas through cell cycle-regulated U2AF2 RNA splicing. Nat Commun 12:4624 (2021). PubMed: 34330897