Deproteinizing Sample Preparation Kit - TCA (ab204708)
Key features and details
- Assay time: 25 min
- Sample type: Tissue Culture Media, Tissue Extracts, Tissue Lysate, Urine, Whole Blood
Overview
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Product name
Deproteinizing Sample Preparation Kit - TCA
See all Deproteinizing sample kits -
Sample type
Urine, Whole Blood, Tissue Extracts, Tissue Culture Media, Tissue Lysate -
Assay time
0h 25m -
Product overview
Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.
This kit is useful where endogenous enzymes and other proteins would interfere with the assay, or would act directly to affect the level of the analyte to be measured, or would bind the analyte.
In this kit, trichloroacetic acid (TCA) is used to precipitate proteins by drastically lowering the sample pH. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. Small molecules present in the sample can then be easily analyzed without interference from endogenous enzymes and other proteins .
The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K823 Deproteinizing Sample Preparation Kit II. K823-200 is the same size as the 200 test size of ab204708.
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Tested applications
Suitable for: Purificationmore details
Properties
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Storage instructions
Store at room temperature. Please refer to protocols. -
Components 200 tests Neutralization Buffer I 1 x 4ml Trichloroacetic Acid Solution 1 x 3ml -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab204708 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Purification |
Use at an assay dependent concentration.
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Notes |
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Purification
Use at an assay dependent concentration. |
Images
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Effect of TCA amount on deproteinization:
Aliquots of pooledhuman serum (off the clot, 100 μL) were treated with different amounts of TCA following the kit protocol. Estimated protein concentration before deproteinization: 7.9 g/dL. More than 99% of protein was removed using less than 10 μL of TCA/100 μL human serum. Red solid line = Total protein; blue dotted line = sample treated with TCA; green solid line = sample treated with spin column.
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Comparison of analysis of lactate:
Human serum samples (100 μL) were analyzed for lactate concentration using L-Lactate Assay Kit (Colorimetric) (ab65331). Untreated samples (purple line) show higher signal when compared to TCA treated samples (red line). However, background signal is very similar in both untreated (green line) and treated (blue line) samples.
Datasheets and documents
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SDS download
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Datasheet download
References (57)
ab204708 has been referenced in 57 publications.
- Harrison MAA et al. Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the Microenvironment. Viruses 14:N/A (2022). PubMed: 35062307
- Oliveira AL et al. Enhanced RAGE Expression and Excess Reactive-Oxygen Species Production Mediates Rho Kinase-Dependent Detrusor Overactivity After Methylglyoxal Exposure. Front Physiol 13:860342 (2022). PubMed: 35418871
- Xu F et al. Blocking autophagy with chloroquine aggravates lipid accumulation and reduces intracellular energy synthesis in hepatocellular carcinoma cells, both contributing to its anti-proliferative effect. J Cancer Res Clin Oncol 148:3243-3256 (2022). PubMed: 35695930
- Upadhyay PR et al. Differential Induction of Reactive Oxygen Species and Expression of Antioxidant Enzymes in Human Melanocytes Correlate with Melanin Content: Implications on the Response to Solar UV and Melanoma Susceptibility. Antioxidants (Basel) 11:N/A (2022). PubMed: 35740103
- Khan MW et al. The hexokinase "HKDC1" interaction with the mitochondria is essential for liver cancer progression. Cell Death Dis 13:660 (2022). PubMed: 35902556